Cetyltrimethylammonium bromide (CTAB)-capped positively-charged silver nanoparticles synthesized in water-ethanol system was electrostatic assembled on predefined aligned X-DNA template. Silver nanowire can be obtained by changing the reaction time and the particles concentration. In our work, the length of the silver nanowire obtained is about 10 mu m, and the dimension of the wires is about 20 nm. AFM data reveal that the assembly of CTAB-capped silver nanoparticles on DNA is ordered, but there is space between two particles absorbed on the DNA template. X-ray photoelectron spectroscopy (XPS) was applied to characterize the linear silver clusters, which provides an additional proof that the silver particles were assembled onto DNA template with fine order. (c) 2005 Elsevier B.V All rights reserved.