Title: Hydrogen peroxide sensor based on horseradish peroxidase immobilized on a silver nanoparticles/cysteamine/gold electrode
Authors: Ren, CB
Song, Yonghai
Li, Z
Zhu, GY # ×
Issue Date: Mar-2005
Publisher: Springer heidelberg
Series Title: Analytical and bioanalytical chemistry vol:381 issue:6 pages:1179-1185
Abstract: A third-generation hydrogen peroxide biosensor was prepared by immobilizing horseradish peroxidase (HRP) on a gold electrode modified with silver nanoparticles. A freshly-cleaned gold electrode was first immersed in a cysteamine-ethanol solution, and then silver nanoparticles were immobilized on the cysteamine monolayer, and finally HRP was adsorbed onto the surfaces of the silver nanoparticles. This self-assemble process was examined via atomic force microscopy (AFM). The immobilized horseradish peroxidase exhibited an excellent electrocatalytic response toward the reduction of hydrogen peroxide. The linear range of the biosensor was 3.3 mu M to 9.4 mM, and the detection limit was estimated to be 0.78 mu M. Moreover, the biosensor exhibited a fast response, high sensitivity, good reproducibility, and long-term stability.
ISSN: 1618-2642
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Imaging and Photonics
× corresponding author
# (joint) last author

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