Title: The structural transition of DNA-Tris(1,10-phenanthroline) cobalt(III) complexes in ethanol-water solution
Authors: Zheng, JP
Li, Z ×
Wu, AG
Zhou, HL
Bai, HY
Song, Yonghai #
Issue Date: Dec-2002
Publisher: Academic press inc elsevier science
Series Title: Biochemical and Biophysical Research Communications vol:299 issue:5 pages:910-915
Abstract: The interaction of DNA with Tris(1,10-phenanthroline) cobalt(III) was studied by means of atomic force microscopy. Changes in the morphologies of DNA complex in the presence of ethanol may well indicate the crucial role of electrostatic force in causing DNA condensation. With the increase of the concentration of ethanol, electrostatic interaction is enhanced corresponding to a lower dielectric constant. Counterions condense along the sugar phosphate backbone of DNA when e is lowered and the phosphate charge density can thus be neutralized to the level of DNA condensation. Electroanalytical measurement of DNA condensed with Co(phen)(3)(3+) in ethanol solution indicated that intercalating reaction remains existing. According to both the microscopic and spectroscopic results, it can be found that no secondary structure transition occurs upon DNA condensing. B-A conformation transition takes place at more than 60% ethanol solution. (C) 2002 Elsevier Science (USA). All rights reserved.
ISSN: 0006-291X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Imaging and Photonics
× corresponding author
# (joint) last author

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