Title: The use of laser microdissection and SELDI-TOF MS in ovarian cancer tissue to identify protein profiles
Authors: Cadron, Isabelle ×
Van Gorp, Toon
Amant, Frédéric
Vergote, Ignace
Moerman, Philippe
Waelkens, Etienne
Daemen, Anneleen
Van de Plas, Raf
De Moor, Bart
Zeillinger, Robert #
Issue Date: Apr-2009
Publisher: Potamitis Press
Series Title: Anticancer Research vol:29 issue:4 pages:1039-1045
Abstract: BACKGROUND: There is a strong need for prognostic biomarkers in ovarian cancer patients due to the heterogeneous responses on current treatment modalities. MATERIALS AND METHODS: This study investigates the feasibility of combining laser microdissection (LMD) and surface enhanced laser desorption ionization-time of flight mass spectrometry (SELDI-TOF MS) in ovarian cancer tissue to obtain protein profiles. RESULTS: Ideal conditions for preparing a protein lysate were determined and subsequently analysed on SELDI-TOF MS. Applying these protocols on tissue of 9 ovarian cancer patients showed different protein profiles between platinum sensitive and resistant patients. CONCLUSION: This shows that combining optimised protocols for LMD with SELDI-TOF MS can be used to obtain discriminatory protein profiles. However, studies with large patient numbers and validation sets are essential to identify reliable biomarkers using this approach.
ISSN: 0250-7005
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Gynaecological Oncology
Translational Cell & Tissue Research
Laboratory of Protein Phosphorylation and Proteomics
ESAT - STADIUS, Stadius Centre for Dynamical Systems, Signal Processing and Data Analytics
Laboratory of Phosphoproteomics (-)
× corresponding author
# (joint) last author

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