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Title: Highlighted generation of fluorescence signals using simultaneous two-color irradiation on Dronpa mutants
Authors: Ando, Ryoko ×
Flors, Cristina
Mizuno, Hideaki
Hofkens, Johan
Miyawaki, Atsushi #
Issue Date: Jun-2007
Publisher: Biophysical Society
Series Title: Biophysical Journal vol:92 issue:12 pages:L97-L99
Abstract: Dronpa absorbs blue light and emits bright green fluorescence. It can also be converted by strong irradiation at 490 nm to a non fluorescent state, which can then be switched back to the original emissive state with irradiation at 400 nm. Through semirandom mutagenesis studies, we have developed two mutants of Dronpa that show efficient photoswitching kinetics. Compared to Dronpa, the mutants can be turned off by blue light more efficiently. Thus, excitation with an argon laser line (488 nm) makes the mutants quickly become dark such that no substantial fluorescence signals can be observed. Excitation with a violet laser diode (405 nm) also produces no fluorescence signals. Simultaneous 488- and 405-nm irradiation, however, results in a rapid oscillation between the two states, thereby keeping the emissive state population large enough to produce sufficiently bright fluorescence signals.
ISSN: 0006-3495
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Imaging and Photonics
Biochemistry, Molecular and Structural Biology Section
× corresponding author
# (joint) last author

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