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Title: On the use of Z-scan fluorescence correlation experiments on giant unilamellar vesicles
Authors: Gielen, Ellen ×
vandeVen, Martin
Margineanu, Anca
Dedecker, Peter
Van der Auweraer, Mark
Engelborghs, Yves
Hofkens, Johan
Ameloot, Marcel #
Issue Date: Feb-2009
Publisher: Elsevier
Series Title: Chemical Physics Letters vol:469 issue:1-3 pages:110-114
Abstract: Z-scan fluorescence correlation spectroscopy (FCS) uses multiple focus planes to determine diffusion coefficients in supported phospholipid bilayers (SPBs) without the need for extrinsic calibration. The approach can be used to discriminate diffusion modes. In this work, Z-scan FCS was applied to study the diffusion of the fluorescent lipid analogue DiI-C-18(5) in the top membrane of giant unilamellar vesicles (GUVs) in the electroformation chamber. In contrast to SPBs, the intrinsic estimate of the radial waist might differ from the one obtained by extrinsic calibration. We describe a pragmatic approach to verify the validity of the Z-scan approach under these conditions. (C) 2008 Elsevier B.V. All rights reserved.
URI: 
ISSN: 0009-2614
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biochemistry, Molecular and Structural Biology Section
Molecular Imaging and Photonics
× corresponding author
# (joint) last author

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