Title: Linking Phospholipase Mobility to Activity by Single-Molecule Wide-Field Microscopy
Authors: Rocha, Susana ×
Hutchison, James Andell
Peneva, Kalina
Herrmann, Andreas
Muellen, Klaus
Skjot, Michael
Jorgensen, Christian I
Svendsen, Allan
De Schryver, Frans
Hofkens, Johan
Uji-I, Hiroshi #
Issue Date: Jan-2009
Publisher: Wiley-v c h verlag gmbh
Series Title: Chemphyschem vol:10 issue:1 pages:151-161
Abstract: Many of the biological processes taking place in cells are mediated by enzymatic reactions occurring in the cell membrane. Understanding interfacial enzymatic catalysis is therefore crucial to the understanding of cellular function. Unfortunately, a full picture of the overall mechanism of interfacial enzymatic catalysis, and particularly the important diffusion processes therein, remains unresolved. Herein we demonstrate that single-molecule wide-field fluorescence microscopy can yield important new information on these processes. We image phospholipase enzymes acting upon bilayers of their natural phospholipid substrate, tracking the diffusion of thousands of individual enzymes while simultaneously visualising local structural changes to the substrate layer. We study several enzyme types with different affinities and catalytic activities towards the substrate. Analysis of the trajectories of each enzyme type allows us successfully to correlate the mobility of phospholipase with its catalytic activity at the substrate. The methods introduced herein represent a promising new approach to the study of interfacial/heterogeneous catalysis systems.
ISSN: 1439-4235
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Imaging and Photonics
× corresponding author
# (joint) last author

Files in This Item:

There are no files associated with this item.

Request a copy


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science