Title: Raman-assisted crystallography reveals end-on peroxide intermediates in a nonheme iron enzyme
Authors: Katona, Gergely
Carpentier, Philippe
Niviere, Vincent
Amara, Patricia
Adam, Virgile
Ohana, Jereny
Tsanov, Nikolay
Bourgeois, Dominique # ×
Issue Date: Apr-2007
Publisher: Amer assoc advancement science
Series Title: Science vol:316 issue:5823 pages:449-453
Abstract: Iron-peroxide intermediates are central in the reaction cycle of many iron-containing biomolecules. We trapped iron(III)-(hydro) peroxo species in crystals of superoxide reductase (SOR), a nonheme mononuclear iron enzyme that scavenges superoxide radicals. X-ray diffraction data at 1.95 angstrom resolution and Raman spectra recorded in crystallo revealed iron-(hydro) peroxo intermediates with the ( hydro) peroxo group bound end-on. The dynamic SOR active site promotes the formation of transient hydrogen bond networks, which presumably assist the cleavage of the iron- oxygen bond in order to release the reaction product, hydrogen peroxide.
ISSN: 0036-8075
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Imaging and Photonics
× corresponding author
# (joint) last author

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