Title: Second-Harmonic Generation in GFP-like Proteins
Authors: Asselberghs, Inge ×
Flors, Cristina
Ferrighi, Lara
Botek, Edith
Champagne, Benoit
Mizuno, Hideaki
Ando, Ryoko
Miyawaki, Atsushi
Hofkens, Johan
Van der Auweraer, Mark
Clays, Koen #
Issue Date: Nov-2008
Publisher: American Chemical Society
Series Title: Journal of the American Chemical Society vol:130 issue:46 pages:15713-15719
Abstract: The second-order nonlinear optical properties of green fluorescent proteins (GFPs), such as the photoswitchable Dronpa and enhanced GFP (EGFP), have been studied at both the theoretical and experimental levels. In the case of Dronpa, both approaches are consistent in showing the rather counterintuitive result of a larger second-order nonlinear polarizability (or first hyperpolarizability, beta) for the protonated state, which has a higher transition energy, than for the deprotonated, fluorescent state with its absorption at lower energy. Moreover, the value of beta for the protonated form of Dronpa is among the highest reported for proteins. In addition to the pH dependence, we have found a wavelength dependence in the beta values. These properties are essential for the practical use of Dronpa or other GFP-like fluorescent proteins as second-order nonlinear fluorophores for symmetry-sensitive nonlinear microscopy imaging and as nonlinear optical sensors for electrophysiological processes. An accurate value of the first hyperpolarizability is also essential for any qualitative analysis of the nonlinear images.
ISSN: 0002-7863
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Imaging and Photonics
Biochemistry, Molecular and Structural Biology Section
× corresponding author
# (joint) last author

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