Title: Where is TRPV1 expressed in the bladder, do we see the real channel?
Authors: Everaerts, Wouter ×
Sepulveda, M. Rosario
Gevaert, Thomas
Roskams, Tania
Nilius, Bernd
De Ridder, Dirk #
Issue Date: Apr-2009
Publisher: Springer-Verlag Heidelberg
Series Title: Naunyn-Schmiedebergs Archives of Pharmacology vol:379 issue:4 pages:421-425
Abstract: Transient receptor potential channel-vanilloid subfamily member 1 (TRPV1) is an important target in the treatment of bladder overactivity. This receptor is suggested to function as a mechanosensor in the normal bladder and to mediate the development of bladder overactivity during cystitis. Our aim was to determine the cellular distribution of TRPV1 in mouse and rat bladder tissue. We used three different commercial TRPV1 antibodies to perform immunohistochemistry on bladder tissue from rats and wildtype and TRPV1(-/-) mice, using trigeminal ganglia as a control tissue for TRPV1 expression. Although two of the antibodies seemed to react specifically in trigeminal ganglion tissue, all the antibodies produced a similar staining pattern in the urothelium of wild-type and TRPV1(-/-) mice. These data show that TRPV1 antibodies can cause an aspecific immunostaining in bladder tissue, urging for additional research to confirm the exact distribution of TRPV1 in bladder. In conclusion, we think that the use of negative controls on knockout mice, whenever available, is mandatory when conducting immunohistochemical localization studies.
ISSN: 0028-1298
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Ion Channel Research
Urology Section (-)
Screening, Diagnostics and Biomarkers (-)
Translational Cell & Tissue Research
Department of Cellular and Molecular Medicine - miscellaneous
× corresponding author
# (joint) last author

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