Title: Plectin deficiency affects precursor formation and dynamics of vimentin networks
Authors: Spurny, Radovan ×
Gregor, Martin
Castanon, Maria J
Wiche, Gerhard #
Issue Date: Nov-2008
Publisher: Elsevier inc
Series Title: Experimental Cell Research vol:314 issue:19 pages:3570-3580
Abstract: Plectin is a typical cytolinker protein that connects intermediate filaments to the other cytoskeietal filament systems and anchors them at membrane-associated junctional sites. One of the most important binding partners of plectin in Fibroblasts is the intermediate filament subunit protein vimentin. Previous studies have demonstrated that vimentin networks are highly dynamic structures whose assembly and disassembly is accomplished stepwise via several intermediates. The precursor forms as well as polymerized (filamentous) vimentin are found in the cells in a dynamic equilibrium characterized by the turnover of the subunits within the polymer and the movement of the smaller precursors. To examine whether plectin plays a role in intermediate filament dynamics, we studied vimentin filament formation in plectin-deficient compared to wildtype fibroblasts using GFP-tagged vimentin. Monitoring vimentin and plectin in spreading and dividing cells, we demonstrate that plectin is associated with vimentin from the early stages of assembly and is required for vimentin motility as well as for the stepwise formation of stable filaments. Furthermore, plectin prevents vimentin networks from complete disassembly during mitosis, facilitating the rebuilding of the intermediate filament network in daughter cells. (C) 2008 Elsevier Inc. All rights reserved.
ISSN: 0014-4827
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Structural Neurobiology
× corresponding author
# (joint) last author

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