Comparison of three ELISAs for the routine diagnosis of human T-lymphotropic virus infection in a high-prevalence setting in Peru
Verdonck, Kristien × González, Elsa Maldonado, Fernando Agapito, Doris Van Dooren, Sonia Vandamme, Anne-Mieke Silva-Santisteban, Alfonso Vanham, Guido Clark, Daniel Gotuzzo, Eduardo #
Transactions of the Royal Society of Tropical Medicine and Hygiene vol:103 issue:4 pages:420-422
To compare three human T-lymphotropic virus (HTLV) ELISAs in a high-prevalence setting, we recruited 300 adults: 125 relatives of HTLV-infected subjects and 175 patients with possible diagnoses of HTLV-associated diseases. Sera were tested with Platelia, Murex, and Ortho ELISA. Samples with positive or discordant ELISA underwent confirmatory Inno-Lia testing. Inno-Lia gave 85/300 HTLV-1-positive and 1/300 HTLV-2-positive results. The positive predictive value was 98% for Platelia, and 100% for Murex and Ortho. Six samples had discordant ELISA; Murex gave one false-negative result, Ortho two and Platelia one. In high-prevalence settings, it is recommended to test samples with two ELISAs before considering them HTLV-seronegative.