Effects of homocysteine on the cellular responses of murine endothelial cells cultured in vitro
Tacheny, A × dos Santos, S Huet, A-C van Steenbrugge, M Michiels, C Holvoet, Paul Raes, M #
Annals of the New York Academy of Sciences vol:973 pages:550-4
Homocysteine, derived from the metabolism of methionine, is claimed as a proatherogenic factor that leads to vascular dysfunction. To gain better insight into the molecular mechanisms involved, homocysteine was tested on a model of murine endothelial cells cultured in vitro, using a prototype DNA chip. The DNA chip was designed to follow the expression at the mRNA level of some major proinflammatory genes; TNF-alpha was used as a positive control.