Title: C-13 NMR evidence of the failure of human erythrocytes to metabolize ascorbate and dehydroascorbate to lactate
Authors: Himmelreich, Uwe ×
Emling, M
Drew, KN
Serianni, AS
Kuchel, PW #
Issue Date: Jun-2000
Publisher: Pergamon-elsevier science ltd
Series Title: Free radical biology and medicine vol:28 issue:11 pages:1607-1610
Abstract: C-13-NMR spectroscopy was used to record time courses of the metabolism of [1-C-13]-L-ascorbic acid (AA) and [2-C-13]-L-ascorbic acid and their dehydro-counterparts (DHAA) by human erythrocytes. Under a range of experimental conditions, but most notably in the absence of glucose in the incubation medium, no C-13-NMR signal for lactate emerged during any of the 5 h time courses. The NMR resonances that did emerge over time were assigned to diketogulonic (DKG) acid and CO2. Only very minor resonances from degradation products of DKG appeared from samples that contained physiologically high concentrations of DHAA. These results are in contrast with those in a recent report that lactate is derived from AA in human erythrocytes. However, an explanation for this possible artifact is given. (C) 2000 Elsevier Science Inc.
ISSN: 0891-5849
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Radiology
Biomedical MRI
× corresponding author
# (joint) last author

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