Title: Cloning of neuropeptide-like precursor 1 in the grey flesh fly and peptide identification and expression
Authors: Verleyen, Peter ×
Chen, X.
Baron, Szilvia
Preumont, A.
Hua, Y-J
Schoofs, Liliane
Clynen, Elke #
Issue Date: 2009
Publisher: Elsevier
Series Title: Peptides vol:30 issue:3 pages:522-530
Abstract: The neuropeptide-like precursor 1 (NPLP1) was first identified in a peptidomics experiment on Drosophila melanogaster. Limited data on this novel neuropeptide precursor suggest a role in the regulation of ecdysis in holometabolous larvae. In this study, we characterized the NPLP1 precursor in the gray flesh fly, Neobellieria bullata, which is an excellent model for physiological assays and hence to discover the role of the NPLP1 peptides. Antisera against three of the D. melanogaster NPLP1 neuropeptides stained an identical set of neurons in the central nervous system of N. bullata compared to D. melanogaster. A novel approach was applied to identify the N. bullata NPLP1 orthologs. Using a combination of affinity chromatography, mass spectrometry, cDNA cloning and RACE experiments, we obtained almost the complete coding sequence of the NPLP1 mRNA. Three encoded NPLP1 peptides were identified in central nervous system extracts by mass spectrometry. Neither doses of 25–250 pmol of synthetic Neb-MGYamide and Neb-PQNamide peptides, nor the NPLP1 antisera did affect the speed of retraction, contraction and tanning in the pupariation bioassay
ISSN: 0196-9781
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Cellular Transport Systems
Animal Physiology and Neurobiology Section - miscellaneous
× corresponding author
# (joint) last author

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