Title: Characterization of dihydrostreptomycin-related substances by liquid chromatography coupled to ion trap mass spectrometry
Authors: Pendela, Murali Mohan
Hoogmartens, Jos
Van Schepdael, Ann
Adams, Erwin # ×
Issue Date: Jun-2009
Publisher: John wiley & sons ltd
Series Title: Rapid communications in mass spectrometry vol:23 issue:12 pages:1856-1862
Abstract: Dihydrostreptomycin sulphate (DHS) is a water-soluble, broad-spectrum aminoglycoside antibiotic. For quantitative analysis, the European Pharmacopoeia (Ph. Eur.) prescribes an ion-pairing liquid chromatography/ultraviolet (LC/UV) method using a C18 stationary phase. Several unknown compounds were detected in commercial samples. Hence, for characterization of these unknown peaks in a commercial DHS sample, the Ph. Eur. method was coupled to mass spectrometry (MS). However, since the Ph. Eur. method uses a non-volatile mobile phase, each peak eluted was collected and desalted before introduction into the mass spectrometer. The desalting procedure was applied to remove the non volatile salt, buffer and ion-pairing reagent in the collected fraction. In total, 20 impurities were studied and 14 of them were newly characterized. Five impurities which are already reported in the literature were also traced in this LC/UV method. Copyright (C) 2009 John Wiley & Sons, Ltd.
ISSN: 0951-4198
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Pharmaceutical Analysis
× corresponding author
# (joint) last author

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