Title: Multimodal imaging of neural progenitor cell fate in rodents
Authors: Waerzeggers, Yannic ×
Klein, Markus
Miletic, Hrvoje
Himmelreich, Uwe
Li, Hongfeng
Monfared, Parisa
Herrlinger, Ulrich
Hoehn, Mathias
Coenen, Heinrich Hubert
Weller, Michael
Winkeler, Alexandra
Jacobs, Andreas Hans #
Issue Date: Mar-2008
Publisher: BC Decker
Series Title: Molecular Imaging vol:7 issue:2 pages:77-91
Abstract: For clinical application of stem cell-based therapies, noninvasive detection of applied stem cells is of high importance. We report on the feasibility of detecting implanted neural progenitor cells (NPCs) noninvasively and follow their fate and functional status by sequential multimodal molecular imaging and reporter gene technology. We investigated C17.2 cells stably expressing herpes simplex virus type 1-thymidine kinase (HSV-1-tk) and green fluorescent protein (gfp) (C17.2-tkIRESgfp = C17.2-TIG) or HSV-1-tk, gfp, and firefly luciferase (luc) (C17.2-lucIREStkgfp = C17.2-LITG) and determined the detection sensitivity of positron emission tomography (PET) and bioluminescence imaging (BLI) for these cells in culture and in vivo in subcutaneous and intracranial glioma models. In addition, PET and BLI were used to further investigate and follow the fate of implanted C17.2-LITG cells in an intracranial glioma model. We show that both imaging modalities are sensitive in detecting reporter gene expressing NPCs; however, PET, by the use of 9-[4-[F-18]fluoro-3-hydroxymethyl)butyl]guanine ([F-18]FHBG), detects NPCs only at sites of disrupted blood-brain barrier. Furthermore, both imaging modalities can be used to detect stem cell fate and migration and indicate excessive proliferation and aberrant migration. In conclusion, multimodal imaging can be used for longitudinal noninvasive monitoring of grafted NPCs in rodents.
ISSN: 1535-3508
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Radiology
Biomedical MRI
× corresponding author
# (joint) last author

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