Title: Immunohistochemically detected ontogeny of prolactin and growth hormone cells in the African catfish Clarias gariepinus
Authors: Volckaert, Filip ×
Mugoyo, James
Lescroart, Olivier
Grisez, Luc
Ollevier, Frans #
Issue Date: 1999
Publisher: Pergamon
Series Title: Comparative biochemistry and physiology. B. Comparative biochemistry vol:122 pages:423-431
Abstract: The chronological appearance of selected endocrine cells in the pituitary of African catfish Clarias gariepinus (Burchell, 1822) was studied morphologically, histologically and immunohistochemically by using antisera raised against catfish growth hormone (cgGH) and recombinant tilapia prolactin I (tPRL). cgGH- and tPRL-like immunoreactive cells were visible from day 1 post fertilisation (hatching) throughout the juvenile and the adult stage. From I to 90 days after hatching, the larval pituitary is oval in shape with a distinctly shaped rostral pars distalis, proximal pars distalis and pars intermedia. From day 120 onwards allometric growth of the rostral and proximal pars distalis extended the prolactin and growth hormone cells anteriorily and posteriorily, respectively. Size and activity of the prolactin and growth hormone cells: measured by the ratio of cell surface to nuclear surface remained constant until day 40 and showed a growth spurt thereafter. Growth hormone content, measured with a catfish-specific radio-immunoassay from hatching until 60 h post hatching, increased exponentially between 30 and 60 h.
ISSN: 0305-0491
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Ecology, Evolution and Biodiversity Conservation Section
Intellectual Property
× corresponding author
# (joint) last author

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