Synthetic and biogenic magnetic nanoparticles for tracking of stem cells and dendritic cells
Schwarz, Sebastian Fernandes, Fabiana Sanroman, Laura Hodenius, Michael Lang, Claus Himmelreich, Uwe Schmitz-Rode, Thomas Schueler, Dirk Hoehn, Mathias Zenke, Martin Hieronymus, Thomas # ×
North-Holland Pub. Co.
Journal of Magnetism and Magnetic Materials vol:321 issue:10 pages:1533-1538
Accurate delivery of cells to target organs is critical for success of cell-based therapies with stem cells or
immune cells such as antigen-presenting dendritic cells (DC). Labeling with contrast agents before
implantation provides a powerful means for monitoring cellular migration using magnetic resonance
imaging (MRI). In this study, we investigated the uptake of fully synthesized or bacterial magnetic
nanoparticles (MNPs) into hematopoietic Flt3+ stem cells and DC from mouse bone marrow. We show
that (i) uptake of both synthetic and biogenic nanoparticles into cells endow magnetic activity and
(ii) low numbers of MNP-loaded cells are readily detected by MRI.
2009 Elsevier B.V. All rights reserved.