Title: Directed differentiation of mouse cochlear neural progenitors in vitro
Authors: Lin, Jizhen ×
Feng, Ling
Hamajima, Yuki
Komori, Masahiro
Burns, Terry C
Fukudome, Shinji
Anderson, John
Wang, Dong
Verfaillie, Catherine
Low, Walter C #
Issue Date: Mar-2009
Series Title: American Journal of Physiology. Cell Physiology vol:296 issue:3 pages:C441-C452
Abstract: Multipotent cochlear neural progenitors (CNPs) in the organ of Corti hold the promise for cell replacement in degenerative hearing disorders. However, not much is known about the CNPs and the specific conditions for their differentiation. Here we isolate the CNPs from the postnatal day 1 organ of Corti in mice and demonstrate their capability to self-renew and to differentiate into hair cell-like and neuronal cell-like phenotypes under the guidance of sonic hedgehog (SHH), epidermal growth factor (EGF), retinoic acid (RA), and brain-derived neurotrophic factor (BDNF), herein termed SERB (abbreviation of SHH, EGF, RA, and BDNF) in an asymmetric or symmetric manner from clonal isolates. Differentiation of CNPs into hair cells by SERB was dependent on the ERK signaling pathway, whereas the differentiation of CNPs into neurons by SERB was not. This work develops a new in vitro methodology for the maintenance and self-regeneration of CNPs for future design of regenerative strategies for hearing disorders.
ISSN: 0363-6143
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Interdepartemental Stem Cell Institute (-)
× corresponding author
# (joint) last author

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