Title: A fast and sensitive method for measuring the integrity of siRNA-carrier complexes in full human serum
Authors: Buyens, Kevin ×
Lucas, Bart
Raemdonck, Koen
Braeckmans, Kevin
Vercammen, Jo
Hendrix, Jelle
Engelborghs, Yves
De Smedt, Stefaan C
Sanders, Niek N #
Issue Date: Feb-2008
Publisher: Elsevier Science Publishers
Series Title: Journal of Controlled Release vol:126 issue:1 pages:67-76
Abstract: SiRNA based therapeutics are currently under investigation for treatment of cancer and viral infections. Upon intravenous administration, the nanoscopic delivery systems which carry the siRNA need to be stable in serum, an aspect which is often overlooked in numerous publications on siRNA delivery systems. Techniques currently available for studying the dissociation of siRNA-liposome complexes are time consuming and incompatible with full serum. We therefore developed a fluorescence fluctuation spectroscopy (FFS) based method which allows to monitor the integrity of siRNA-carrier complexes. The method can very rapidly provide quantitative information on the complex integrity in biological media, like full human serum, and at very low siRNA concentrations (approximately 20 nM siRNA). Information on the integrity of intravenously injected siRNA nanoparticles in serum is crucial. Consequently, the FFS method reported in this work may find broad applicability in the field of siRNA-carrier design.
ISSN: 0168-3659
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biochemistry, Molecular and Structural Biology Section
× corresponding author
# (joint) last author

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