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Title: TRPC1 channels regulate directionality of migrating cells
Authors: Fabian, A
Fortmann, T
Dieterich, P
Riethmueller, C
Schoen, P
Mally, S
Nilius, Bernd
Schwab, A # ×
Issue Date: Nov-2008
Publisher: Springer-Verlag
Series Title: Pflügers Archiv: European Journal of Physiology vol:457 issue:2 pages:475-484
Abstract: Cell migration depends on the generation of structural asymmetry and on different steps: protrusion and adhesion at the front and traction and detachment at the rear part of the cell. The activity of Ca2+ channels coordinate these steps by arranging intracellular Ca2+ signals along the axis of movement. Here, we investigated the role of the putative mechanosensitive canonical transient receptor potential channel 1 (TRPC1) in cell migration. We analyzed its function in transformed renal epithelial (Madin-Darby canine kidney-focus) cells with variation of TRPC1 expression. As shown by time lapse video microscopy, TRPC1 knockdown cells have partially lost their polarity and the ability to persistently migrate into a given direction. This failure is linked to the suppression of a local Ca2+ gradient at the front of migrating TRPC1 knockdown cells, whereas TRPC1 overexpression leads to steeper Ca2+ gradients. We propose that the Ca2+ signaling events regulated by TRPC1 within the lamellipodium determine polarity and directed cell migration.
ISSN: 0031-6768
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Ion Channel Research
Department of Cellular and Molecular Medicine - miscellaneous
× corresponding author
# (joint) last author

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