Title: TRPM4 regulates migration of mast cells in mice
Authors: Shimizu, Takahiro *
Owsianik, Grzegorz *
Freichel, Marc
Flockerzi, Veit
Nilius, Bernd ×
Vennekens, Rudi #
Issue Date: Mar-2009
Publisher: Churchill livingstone
Series Title: Cell Calcium vol:45 issue:3 pages:226-232
Abstract: We demonstrate here that the transient receptor potential melastatin subfamily channel, TRPM4, controls migration of bone marrow-derived mast cells (BMMCs), triggered by dinitrophenylated human serum albumin (DNP-HSA) or stem cell factor (SCF). Wild-type BMMCs migrate after stimulation with DNP-HSA or SCIF whereas both stimuli do not induce migration in BMMCs derived from TRPM4 knockout mice (trpm4(-/-)). Mast cell migration is a Ca2+-dependent process, and TRPM4 likely controls this process by setting the intracellular Ca2+ level upon cell stimulation. Cell migration depends on filamentous actin (F-actin) rearrangement, since pretreatment with cytochalasin B, an inhibitor of F-actin formation, prevented both DNP-HSA- and SCIF-incluced migration in wild-type BMMC. Immunocytochemical experiments using fluorescence-conjugated phalloidin demonstrate a reduced level of F-actin formation in DNP-HSA-stimulated BMMCs from trpm4(-/-) mice. Thus, our results suggest that TRPM4 is critically involved in migration of BMMCs by regulation of Ca2+-dependent actin cytoskeleton rearrangements. (c) 2008 Elsevier Ltd. All rights reserved.
ISSN: 0143-4160
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Ion Channel Research (VIB-KU Leuven Center for Brain & Disease Research)
Physiology Section (-)
Department of Cellular and Molecular Medicine - miscellaneous
* (joint) first author
× corresponding author
# (joint) last author

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