Author:

Keywords:

Science & Technology, Life Sciences & Biomedicine, Microbiology, Pseudomonas aeruginosa, phage phi KMV, type IV pili, FIBROSIS LUNG INFECTION, CYSTIC-FIBROSIS, DIFFERENTIAL REGULATION, TWITCHING MOTILITY, ESCHERICHIA-COLI, GENOMIC ANALYSIS, PHAGE, HOST, MECHANISMS, FREQUENCY, Fimbriae, Bacterial, Gene Library, Genes, Bacterial, Genetic Complementation Test, Locomotion, Pseudomonas Phages, Virus Attachment, 06 Biological Sciences, 07 Agricultural and Veterinary Sciences, 11 Medical and Health Sciences, 30 Agricultural, veterinary and food sciences, 31 Biological sciences, 32 Biomedical and clinical sciences

Abstract:

Abstract Pseudomonas aeruginosa bacteriophage phiKMV requires type IV pili for infection, as observed from the phenotypic characterization and phage adsorption assays on a phage infection-resistant host strain mutant. A cosmid clone library of the host (P. aeruginosa PAO1) genomic DNA was generated and used to select for a clone that was able to restore phiKMV infection in the resistant mutant. This complementing cosmid also re-established type IV pili-dependent twitching motility. The correlation between bacteriophage phiKMV infectivity and type IV pili, along with its associated twitching motility, was confirmed by the resistance of a P. aeruginosa PAO1DeltapilA mutant to the phage. Subcloning of the complementing cosmid and further phage infection analysis and motility assays suggests that a common regulatory mechanism and/or interaction between the ponA and pilMNOPQ gene products are essential for bacteriophage phiKMV infectivity.