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Title: Identification and comparative analysis of the structural proteomes of phiKZ and EL, two giant Pseudomonas aeruginosa bacteriophages
Authors: Lecoutere, Elke
Ceyssens, Pieter-Jan
Miroshnikov, Konstantin A
Mesyanzhinov, Vadim V
Krylov, Victor N
Noben, Jean-Paul
Robben, Johan
Hertveldt, Kirsten
Volckaert, Guido
Lavigne, Rob # ×
Issue Date: Jun-2009
Series Title: Proteomics vol:9 issue:11 pages:3215-3219
Abstract: Giant bacteriophages phiKZ and EL of Pseudomonas aeruginosa contain 62 and 64 structural proteins, respectively, identified by ESI-MS/MS on total virion particle proteins. These identifications verify gene predictions and delineate the genomic regions dedicated to phage assembly and capsid formation (30 proteins were identified from a tailless phiKZ mutant). These data form the basis for future structural studies and provide insights into the relatedness of these large phages. The phiKZ structural proteome strongly correlates to that of Pseudomonas chlororaphis bacteriophage 201phi2-1. Phage EL is more distantly related, shown by its 26 non-conserved structural proteins and the presence of genomic inversions.
URI: 
ISSN: 1615-9853
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Gene Technology (-)
Biochemistry, Molecular and Structural Biology Section
× corresponding author
# (joint) last author

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