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Title: Ischaemic ATP degradation studied by HPLC and 31P-NMR spectroscopy: do the two techniques observe the same ATP pools?
Authors: Herijgers, Paul ×
Overloop, K
Toshima, Y
Van Hecke, Paul
Vanstapel, Florent
Mubagwa, Kanigula
Flameng, Willem #
Issue Date: Feb-1994
Series Title: Basic research in cardiology vol:89 issue:1 pages:50-60
Abstract: 31P-NMR spectroscopy has become the major tool for studying myocardial high energy phosphates. Conflicting results concerning NMR visibility of ATP in ischaemic myocardium were reported. A detailed study was undertaken to resolve this controversy. After cardioplegic arrest, canine hearts were excised and preserved for 24 h at 1 degree C (group 1) or for 6h at 23 degrees C (group 2). ATP breakdown was followed by 31P-NMR spectroscopy in a transmural piece of the anterior wall introduced in the NMR magnet, and by HPLC analysis using serial transmural biopsies from the rest of the anterior wall. At both temperatures, identical relative ATP decay curves were obtained, whether measured by NMR or by HPLC. Absolute quantification of ATP was carried out after varying periods of ischaemia at 1 degree C. The NMR-measured ATP concentration was 106 +/- 8% of the ATP concentration determined by HPLC. From our experiments, we conclude that ATP visibility for 31P-NMR spectroscopy is complete and constant during prolonged periods of hypothermic ischaemia in canine hearts.
ISSN: 0300-8428
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Experimental Cardiac Surgery (-)
Laboratory of Clinical Bacteriology and Mycology
Biomedical Quality Assurance Research Unit
× corresponding author
# (joint) last author

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