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Title: Relationship of lipoprotein-associated phospholipase A2 and oxidized low-density lipoprotein in carotid atherosclerosis
Authors: Vickers, Kasey C ×
Maguire, Colin T
Wolfert, Robert
Burns, Alan R
Reardon, Michael
Geis, Richard
Holvoet, Paul
Morrisett, Joel D #
Issue Date: Sep-2009
Publisher: American Society for Biochemistry and Molecular Biology
Series Title: Journal of Lipid Research vol:50 issue:9 pages:1735-1743
Abstract: Plasma levels of Lp-PLA2 and oxLDL have been identified as risk factors for cardiovascular disease. Lp-PLA2 is the sole enzyme responsible for the hydrolysis of oxidized phospholipids on LDL particles in atherosclerotic plaques. We have studied the relationship between Lp-PLA2 and oxLDL in carotid endarterectomy (CEA) tissues and in matched plasmas. In extracts from CEA anatomical segments the levels of oxLDL were significantly associated with the levels of Lp-PLA2 protein (r=0.497) and activity (r=0.615). OxLDL and Lp-PLA2 mass/activity were most abundant in the carotid bifurcation and internal segments where plaque was most abundant. In extracts from CEA atheroma, the levels of oxLDL and Lp-PLA2 were significantly correlated (r=0.6346). In matched plasma and atheroma extracts, the levels of Lp-PLA2 were negatively correlated (r= -0.578). The ratio of Lp-PLA2 to oxLDL was higher in atheromatous tissue (277:1) than in normal tissue (135:1) and plasma (13:1). Immunohistochemical experiments indicated that in plaques, oxLDL and Lp-PLA2 existed in overlapping but distinctly different distribution. Fluorescence microscopy showed both oxLDL and Lp-PLA2 epitopes on the same LDL particle in plasma but not in plaque. These results suggest that the relationship between Lp-PLA2 and oxLDL in the atherosclerotic plaque is different from that in the plasma compartment.
URI: 
ISSN: 0022-2275
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Atherosclerosis and Metabolism (-)
× corresponding author
# (joint) last author

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