Maturation of the Translation Inhibitor Microcin C
Metlitskaya, Anastasia Kazakov, Teymur Vondenhoff, Gaston Novikova, Maria Shashkov, Alexander Zatsepin, Timofei Semenova, Ekaterina Zaitseva, Natalia Ramensky, Vasily Van Aerschot, Arthur × Severinov, Konstantin #
American Society for Microbiology (ASM)
Journal of Bacteriology vol:191 issue:7 pages:2380-2387
Microcin C (McC), an inhibitor of the growth of enteric bacteria, consists of a heptapeptide with a modified AMP residue attached to the backbone of the C-terminal aspartate through an N-acyl phosphamidate bond. Here we identify maturation intermediates produced by cells lacking individual mcc McC biosynthesis genes. We show that the products of the mccD and mccE genes are required for attachment of a 3-aminopropyl group to the phosphate of McC and that this group increases the potency of inhibition of the McC target, aspartyl-tRNA synthetase.