ESB 2008 edition:16th congress location:Lucerne, Switzerland date:6-9 july 2008
As part of a bone tissue engineering therapy cell- seeded scaffolds can be cultured in perfusion bioreactors prior to in vivo implantation in the bone defect. The flow mediated mechanical stimuli inside the scaffold appear to be a factor that affects the in vitro proliferation and osteogenic differentiation. Quantification of the fluid dynamic microenvironment inside scaffolds is needed in order to assess this effect. The goal of this study was to develop a combined computational and experimental approach to characterise the fluid flow within porous scaffolds.Results Both experimental and CFD calculations show similar flow patterns, with a peak velocity of 0.4 mm/s.The coupling of experimental and numerical analysis provides a complete flow characterisation and represents the basis to quantify the relation between the hydrodynamic environment and cell growth and differentiation within bone scaffolds.