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Title: Heterogeneous Nuclear Ribonucleoprotein H1, a Novel Nuclear Autoantigen
Authors: Van den Bergh, Karolien ×
Hooijkaas, Herbert
Blockmans, Daniel Engelbert
Westhovens, Rene
Op De Be├ęck, Katrijn
Verschueren, Patrick
Dufour, Diana
van de Merwe, Joop P
Fijak, Monika
Klug, Jörg
Michiels, Georges
Devogelaere, Benoit
De Smedt, Humbert
Derua, Rita
Waelkens, Etienne
Blanckaert, Norbert
Bossuyt, Xavier #
Issue Date: May-2009
Publisher: P.B. Hoeber
Series Title: Clinical Chemistry vol:55 issue:5 pages:946-954
Abstract: BACKGROUND: Serum samples from patients with autoimmune connective tissue diseases that show a finely speckled antinuclear antibody (ANA) on indirect immune-fluorescence often have antibodies against unknown nuclear target antigens. To search for such autoantigens we applied a proteomic approach using sera from patients with a high ANA titer (>or=640) and finely speckled fluorescence but in whom no antibodies to extractable nuclear antigens (ENA) could be identified. METHODS: Using an immunoproteomics approach we identified heterogeneous nuclear ribonucleoprotein H1 (hnRNP H1) as a novel nuclear target of autoantibody response. RESULTS: Recombinant rat hnRNP H1 reacted in Western blot analyses with 48% of 93 sera from patients with primary Sjögren syndrome and with 5.2% of 153 sera from patients with other connective tissue diseases (diseased controls). For comparison, the diagnostic sensitivity and specificity of anti-Sjögren syndrome A (SSA) antibodies for primary Sjögren syndrome in the same patient cohort were 88.2% and 76.3%, respectively. Interestingly, 5 of 11 primary Sjögren syndrome patients with no anti-SSA or anti-SSB antibodies had anti-hnRNP H1 antibodies. Anti-hnRNP H1 antibodies were preabsorbed by hnRNP H1, as demonstrated by indirect immunofluorescence. In an evaluation of the presence of anti-hnRNP H1 antibodies in 188 consecutive samples submitted to the clinical laboratory with positive ANA (titer >or=160), anti-hnRNP H1 antibodies were found in 3 of 7 (2 primary and 5 secondary) Sjögren syndrome patients and in 8.3% of the diseased controls. CONCLUSIONS: HnRNP H1 is a newly discovered autoantigen that could become an additional diagnostic marker.
URI: 
ISSN: 0009-9147
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Molecular and Cellular Signaling
Laboratory for Clinical Infectious and Inflammatory Disorders
Laboratory of Protein Phosphorylation and Proteomics
Laboratory of Clinical Bacteriology and Mycology
Rheumatology Section (-)
Laboratory of Phosphoproteomics (-)
Experimental Laboratory Immunology
Department of Imaging & Pathology - miscellaneous
× corresponding author
# (joint) last author

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