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Title: Oropharyngeal aspiration: An alternative route for challenging in a mouse model of chemical-induced asthma
Authors: De Vooght, Vanessa ×
Vanoirbeek, Jeroen
Haenen, Steven
Verbeken, Eric
Nemery, Benoit
Hoet, Peter #
Issue Date: Mar-2009
Publisher: Elsevier
Series Title: Toxicology vol:259 issue:1-2 pages:84-89
Abstract: Background: To assess the importance of the route of challenge in an existing mouse model of chemicalinduced
asthma, we replaced intranasal instillation by oropharyngeal aspiration. To our knowledge,
oropharyngeal aspiration as a challenge route has not yet been investigated in a mouse model of chemicalinduced
asthma.
Methods:Ondays 1 and 8, micewere dermally sensitized with toluene diisocyanate (TDI) (0.3%) [or vehicle
(acetone/olive oil)] and on day 15 they received a single challenge, via oropharyngeal aspiration, with TDI
(0.01%) or vehicle. One day after challenge, airway reactivity to methacholine was measured by a forced
oscillation technique (FlexiVent) and total and differential cell counts, as well as levels of KC, IL-5, IL-17
and TNF-a, were assessed in the bronchoalveolar lavage (BAL) fluid. Lymphocytes from the auricular and
mediastinal lymph nodeswere cultured to determine the concanavaline A-induced secretion of IL-2, IL-4,
IL-10, IL-13, IL-17 and IFN-g. Total serum IgE was measured.
Results: In TDI-sensitized mice, a significant increase in airway reactivitywas found after a single oropharyngeal
challenge with TDI. BAL neutrophils and eosinophils were increased 7- and 5-fold, respectively.
An upregulation of Th1 (IFN-g), Th2 (IL-4, IL-10, IL-13) and Th17 (IL-17) cytokines was found in the auricular
lymph nodes, in the mediastinal lymph nodes only IL-4 was upregulated. The total serum IgE level
in TDI-sensitized mice was significantly increased when compared to control mice.
Conclusion: We conclude that challenging mice via oropharyngeal aspiration mimics the characteristics
of human asthma well, without the possible drawbacks of other techniques.
ISSN: 0300-483X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Occupational, Environmental and Insurance Medicine (-)
Translational Cell & Tissue Research
Environment and Health - miscellaneous
× corresponding author
# (joint) last author

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