Title: Size-Dependent Cytotoxicity of Monodisperse Silica Nanoparticles in Human Endothelial Cells
Authors: Napierska, Dorota ×
Thomassen, Leen
Rabolli, Virginie
Lison, Dominique
Gonzalez, Laetitia
Kirsch-Volders, Micheline
Martens, Johan
Hoet, Peter #
Issue Date: Apr-2009
Publisher: Wiley
Series Title: Small vol:5 issue:7 pages:846-853
Abstract: The effect that monodisperse amorphous spherical silica particles of different sizes have on the viability of endothelial cells (EAHY926 cell line) is investigated. The results indicate that exposure to silica nanoparticles causes cytotoxic damage (as indicated by lactate dehydrogenase (LDH) release) and a decrease in cell survival (as determined by the tetrazolium reduction, MTT, assay) in the EAHY926 cell line in a dose-related manner. Concentrations leading to a 50% reduction in cell viability (TC50) for the smallest particles tested (14-, 15-, and 16-nm diameter) ranging from, 33 to 47 mu g cm(-2) of cell culture differ significantly from values assessed for the bigger nanoparticles: 89 and 254 mu g cm(-2) (diameter of 19 and 60 mn, respectively). Two fine silica particles with diameters of 104 and 335 nm show very low cytotoxic response compared to nanometer-sized particles with TC50 values of 1095 and 1087 mu g cm(-2), respectively. The smaller particles also appear to affect the exposed cells faster with cell death (by necrosis) being observed within just a few hours. The surface area of the tested particles is an important parameter in determining the toxicity of monodisperse amorphous silica nanoparticles.
ISSN: 1613-6810
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Centre for Surface Chemistry and Catalysis
Occupational, Environmental and Insurance Medicine (-)
Environment and Health - miscellaneous
Formerly "Departement Industriƫle Wetenschappen - TM M"
Microbial and Molecular Systems - miscellaneous
× corresponding author
# (joint) last author

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