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Title: Cloning and expression of the cyclophilin Bet v 7, and analysis of immunological cross-reactivity among the cyclophilin A family
Authors: Cadot, Pascal ×
Nelles, Lucien
Srahna, Mohammed
Dilissen, Ellen
Ceuppens, Jan #
Issue Date: Feb-2006
Series Title: Molecular immunology vol:43 issue:3 pages:226-35
Abstract: Allergic symptoms in sensitized individuals are caused by proteins named allergens. We report here the cloning and the production of the cyclophilin Bet v 7, one of the birch pollen allergens. Recombinant Bet v 7 was produced in bacteria and used to raise a rabbit anti-Bet v 7 antiserum. With this antiserum we detected cyclophilin A in several pollen species and we demonstrated immunological cross-reactivity among those plant cyclophilins A by immunoblot and ELISA inhibition experiments. However, we could not detect cyclophilins in extracts of animal or mould origin with our anti-Bet v 7 antiserum. By inhibition experiments with purified mould cyclophilins, we confirmed the absence of cross-reactivity between plant cyclophilins and non-plant cyclophilins. In addition, our results indicate that the level of immunological cross-reactivity correlates with the level of sequence identity among the cyclophilin A family. This allowed us to define the plant cyclophilin A sub-family as being immunologically distinct, which might have implications at the clinical level in the allergy practice.
ISSN: 0161-5890
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Clinical Immunology
× corresponding author
# (joint) last author

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