Title: Inhibition by a 4-methyl-4-aza-steroid of NADPH: delta 4-3-oxosteroid-5 alpha-oxido-reductase activity in cultured interstitial cells derived from immature rat testis
Authors: Verhoeven, Guido ×
Cailleau, J #
Issue Date: Mar-1983
Series Title: Journal of steroid biochemistry vol:18 issue:3 pages:365-7
Abstract: Prepubertal rat testes have high 5 alpha-reductase (delta 4-3-oxosteroid 5 alpha-oxidoreductase) activity and secrete various 5 alpha-reduced androgens. We demonstrate that Na-4-methyl-4-aza-3-oxo-5 alpha-pregnane-20 (S)-carboxylate, a potent inhibitor of the prostatic 5 alpha-reductase, competitively inhibits the microsomal 5 alpha-reductase of rat testis. The apparent Ki for testosterone is 3.5 nM. At a concentration of 5 X 10(-7) M this compound efficiently inhibits the 5 alpha-reductase in cultured interstitial cells derived from prepubertal rats. Testosterone and 4-androstene-3,17-dione are the major androgens produced by these cells in the presence of inhibitor. It is concluded that 4-methyl-4-aza-steroids are a useful tool in the study of steroid secretion by prepubertal Leydig cells.
ISSN: 0022-4731
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Clinical and Experimental Endocrinology
× corresponding author
# (joint) last author

Files in This Item:

There are no files associated with this item.

Request a copy


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science