Title: Mechanism of the stimulatory effect of phorbol 12-myristate 13-acetate on cellular production of plasminogen activator
Authors: Ashino-Fuse, H ×
Opdenakker, Ghislain
Fuse, A
Billiau, Alfons #
Issue Date: Jun-1984
Series Title: Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.) vol:176 issue:2 pages:109-18
Abstract: The human Bowes melanoma cell line secretes a plasminogen activator identical to the extrinsic tissue plasminogen activator (EPA) and different from the urokinase-like plasminogen activators mostly found in human tumor lines. In the continuous presence of 100 ng/ml of phorbol 12-myristate 13-acetate (PMA) the 24-hr production of EPA was increased 5.3-fold (average). Preincubation of the cultures for a limited time period (optimally 3 to 6 hr) also resulted in an increase of the subsequent 24-hr production. EPA produced in the presence of PMA was serologically indistinguishable from that produced spontaneously and its molecular weight as defined by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis and fibrin-agar zymography was the same as that of spontaneously produced EPA. Treatment of the cells with actinomycin D inhibited PMA-induced EPA production. Also, RNA extracted from PMA-treated cells became enriched in mRNA for EPA. It is concluded that PMA acts by enhancing the transcription of the EPA gene. Cell-associated EPA levels were increased, even when tested as early as 3 hr after initiation of the PMA treatment, thus failing to support the view that increased EPA synthesis occurred as a result of depletion of the cellular pool.
ISSN: 0037-9727
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Immunobiology (Rega Institute)
× corresponding author
# (joint) last author

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