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Title: The effect of systemic antibiotics on the microbiological diagnosis of experimental foreign body infections caused by Staphylococcus epidermidis
Authors: Vandecasteele, Stefaan ×
Van Eldere, Johan
Merckx, Rita
Peetermans, Willy #
Issue Date: Feb-2004
Series Title: Diagnostic microbiology and infectious disease vol:48 issue:2 pages:89-95
Abstract: Qualitative broth culture and quantitative culture on agar were compared with quantitative polymerase chain reaction (PCR) for the diagnosis of foreign body infections (FBI) in a rat model with and without exposure to systemic antibiotics (teicoplanin and rifampin). The 3 methods had a similar and high yield without antibiotics. Antibiotics decreased the number of CFU/foreign body and increased the number of culture-negative foreign bodies and the variability of the results in quantitative culture. The yield of broth culture remained high under antibiotics although prolonged incubation (2-5 days) was required. The yield of the PCR was equivalent or even superior (for teicoplanin) to the yield of broth culture. Quantitative PCR had a higher yield and lower variability than quantitative culture and was not affected by antibiotics. The simultaneous isolation of RNA from all samples indicated viability of the bacteria. Quantitative PCR seems a promising method for the diagnosis of FBI.
URI: 
ISSN: 0732-8893
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Molecular Bacteriology (Rega Institute)
Laboratory for Clinical Infectious and Inflammatory Disorders
Laboratory for Experimental and Clinical Microbiology (-)
Laboratory of Clinical Bacteriology and Mycology
× corresponding author
# (joint) last author

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