Applied Microbiology and Biotechnology vol:82 issue:6 pages:1143-1156
The volumetric productivity of the beer fermentation
process can be increased by using a higher pitching
rate (i.e., higher inoculum size). However, the decreased
yeast net growth observed in these high cell density
fermentations can have a negative impact on the physiological stability throughout subsequent yeast generations.
The use of different oxygen conditions (wort aeration, wort
oxygenation, yeast preoxygenation) was investigated to
improve the growth yield during high cell density fermentations and yeast metabolic and physiological parameters were assessed systematically. Together with a higher extent of growth (dependent on the applied oxygen conditions), the fermentation power and the formation of unsaturated fatty acids were also affected. Wort oxygenation had a significant decreasing effect on the formation of esters, which was caused by a decreased expression of the alcohol acetyl transferase gene ATF1, compared with the other conditions. Lower glycogen and trehalose levels at the end of fermentation were observed in case of the high cell density fermentations with oxygenated wort and the reference fermentation. The expression levels of BAP2 (encoding the branched chain amino acid permease), ERG1 encoding squalene epoxidase), and the stress responsive gene HSP12 were predominantly influenced by the high cell concentrations, while OLE1 (encoding the fatty acid desaturase) and the oxidative stress responsive genes SOD1 and CTT1 were mainly affected by the oxygen availability per cell. These results demonstrate that optimisation of high cell density fermentations could be achieved by improving the oxygen conditions, without drastically affecting the physiological condition of the yeast and beer quality.