Title: GATA-3 in human T cell helper type 2 development
Authors: Skapenko, A ×
Leipe, J
Niesner, U
Devriendt, Koenraad
Beetz, R
Radbruch, A
Kalden, JR
Lipsky, PE
Schulze-Koops, H #
Issue Date: Feb-2004
Publisher: Rockefeller univ press
Series Title: Journal of Experimental Medicine vol:199 issue:3 pages:423-428
Abstract: The delineation of the in vivo role of GATA-3 in human T cell differentiation is a critical step in the understanding of molecular mechanisms directing human immune responses. We examined T cell differentiation and T cell-mediated effector functions in individuals lacking one functional GATA-3 allele. CD4 T cells from GATA-3(+/-) individuals expressed significantly reduced levels of GATA-3, associated with markedly decreased T helper cell (Th)2 frequencies in vivo and in vitro. Moreover, Th2 cell-mediated effector functions, as assessed by serum levels of Th2-dependent immunoglobulins (Igs; IgG4, IgE), were dramatically decreased, whereas the Th1-dependent IgG1 was elevated compared with GATA-3(+/+) controls. Concordant with these data, silencing of GATA-3 in GATA-3(+/+) CD4 T cells with small interfering RNA significantly reduced Th2 cell differentiation. Moreover, GATA-3 mRNA levels increased under Th2-inducing conditions and decreased under Th1-inducing conditions. Taken together, the data strongly suggest that GATA-3 is an important transcription factor in regulating human Th2 cell differentiation in vivo.
ISSN: 0022-1007
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Clinical Genetics Section (-)
Department of Human Genetics - miscellaneous
× corresponding author
# (joint) last author

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