Conference of European Comparative Endocrinologists edition:21 location:Bonn, Germany date:26-30 August 2002
The insect brain-corpus cardiacum complex is the functional equivalent of the hypothalamus-pituitary complex in mammals. In these complexes there is a close collaboration between the neural and the hormonal system. Peptides originating from the brain are stored in the corpora cardiaca until released into the circulation. This study aims at the characterization of the factors regulating this release. For this purpose we developed a perifusion system. Released peptides are trapped on disposable seppak C18 columns and separated on a capillary HPLC system (CapLC). First screenings showed the effects of forskolin, a cAMP activator and biogenic amines such as dopamine, serotonin, tyramine and octopamine. Electrospray ionisation double quadrupole time of flight mass spectrometry is used to identify the released neuropeptides present in the collected CapLC fractions.