Benelux Congress of Zoology edition:7 location:Brussel date:24-25 November
Paralysins are endogenous compounds in immature insects that causes paralysis or death in adult individuals after injection into the thorax. We have proven the universal effect of paralysins by injection of paralysins from Neobellieria bullata into adult Tenebrio molitor and vice versa. The toxic effect of the tested, 30 % ACN fraction from whole body homogenates depends on the extracted stage. The activity of the paralysins shows a temporal distribution with a highest level of activity at pupation and a secondary effect at eclosion.
The dopa decarboxylase (DDC) activity, measured by using a radioactive labeled substrate, in developing Neobellieria bullata en Tenebrio molitor, peaks at the most important moments in the development of the insect: at pupation but especially at eclosion.
The DDC enzyme could play an important role in the toxicity of paralysins. Relating the temporal distribution of toxic activity in both species with their correlated distribution of DDC activity shows the same pattern: DDC activity goes up after stages that show high paralytic activity. After injection of ï¢-alanine-L-tyrosine (BAY, a known paralysin of Neobellieria bullata) into the thorax of adult flies, we can confirm that the DDC activity cannot be induced. So, this could be the key to the toxic effect of BAY, because injection of BAY into the thorax of juvenile (pupae) Tenebrio molitor did cause an induction of DDC activity.