This item still needs to be validated !
Title: Prevention of autoimmune diabetes in NOD mice by 1,25 dihydroxyvitamin D3
Authors: Mathieu, Chantal ×
Waer, Mark
Laureys, Jozef
Rutgeerts, Omer
Bouillon, Roger #
Issue Date: Jun-1994
Publisher: Springer-Verlag Heidelberg
Series Title: Diabetologia vol:37 issue:6 pages:552-558
Abstract: 1,25 dihydroxyvitamin D-3, the active form of vitamin D, has immunomodulatory properties in vitro and in vivo. We report that treatment with 1,25 dihydroxyvitamin D-3 (5 mu g/kg on alternate days) prevents the development of clinical diabetes in NOD mice, an animal model of human autoimmune diabetes. Diabetes incidence in female NOD mice at the age of 200 days was reduced to 8% in the 1,25 dihydroxyvitamin D treated group vs 56% in the control group (p < 0.0001). In parallel, treatment with 1,25 dihydroxyvitamin D-3 resulted in a complete normalisation of the capacity to induce suppressor mechanisms in an autologous MLR, which is severely depressed in control NOD mice. The existence of such suppressor cells was confirmed in transfer experiments, whereby cotransfer of splenocytes from 1,25 dihydroxyvitamin D-3 treated NOD mice prevented diabetes transfer by splenocytes from diabetic NOD mice into irradiated, 6-8-week-old male NOD mice. Other known immune defects of the NOD mice, such as defective natural killer cell killing of YAC-1 targets and defective thymocyte activation by anti-CD3 were not corrected. The pharmacological doses of 1,25 dihydroxyvitamin D-3 were universally well tolerated as reflected by a normal weight gain of the mice. Serum calcium was increased (2.5+/-0.2 vs 2.2+/-0.2 mmol/l in the control group, p < 0.005),whereas osteocalcin levels nearly doubled and bone calcium content was halved. These findings show that 1,25 dihydroxyvitamin D-3 can prevent diabetes in NOD mice, probably through the correction of their defective suppresser function.
ISSN: 0012-186X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Clinical and Experimental Endocrinology
Medical Clerkships Centers
Laboratory of Experimental Transplantation
Laboratory of Nephrology
× corresponding author
# (joint) last author

Files in This Item:

There are no files associated with this item.

Request a copy


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science