Title: Factors influencing the production of human immune interferon components
Authors: Van Damme, Jozef ×
De Ley, Marc
Muylle, L
Billiau, Alfons #
Issue Date: Jan-1983
Series Title: Developments in biological standardization vol:55 pages:135-41
Abstract: Human interferon (IFN), induced in leukocyte suspensions by mitogens, contains two antivirally active components (45K and 22K), separable from each other by gel filtration. The 45K component was identified as IFN-gamma; the 22K component is serologically related to IFN-beta, but has different physicochemical and biological properties. Relatively large amounts of 22K were produced in leukocyte suspensions isolated from buffy coats after induction with concanavalin A (ConA); induction of plateletpheresis residues with staphylococcal enterotoxin A (SEA) resulted in the production of almost only IFN-gamma (45K). The addition of foetal calf serum (FCS) to the production medium enhanced the yields of IFN-gamma, while the production of 22K remained unchanged. Incubation of the cell suspension in the absence of mitogen gave only small amounts of IFN, containing mostly 22K. Under various conditions tested, the two components had similar kinetics of production. A significant increase of IFN-gamma production was observed when leukocytes were primed with low concentrations of 22K.
ISSN: 0301-5149
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Academic Center for General Practice
Laboratory of Molecular Immunology (Rega Institute)
Biochemistry, Molecular and Structural Biology Section
Laboratory of Immunobiology (Rega Institute)
× corresponding author
# (joint) last author

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