Title: Proteome analysis of lymph nodes in a mouse model of chemical-induced asthma
Authors: Haenen, Steven ×
Clynen, Elke
De Vooght, Vanessa
Hoet, Peter
Nemery, Benoit
Vanoirbeek, Jeroen #
Issue Date: 2008
Conference: BelTox Annual Meeting location:Brussels, Belgium date:28 November 2008
Abstract: Introduction: Up to 15% of all asthma cases in adults are due to chemical exposures at the workplace. The identification of biomarkers of sensitization could help to move diagnosis to an earlier stage. Using a mouse model of chemical-induced asthma we will explore the process of sensitization towards chemicals at the protein and peptide level.
Methods: On days 1 and 8, mice are treated on both ears with toluene diisocyanate (TDI, 0.3% or 3%, 20µl), a known respiratory sensitizer, or a mixture of acetone – olive oil (AOO, vehicle-control). On day 15, lymph nodes are collected and homogenized.
We used two-dimensional differential gel electrophoresis (2D-DIGE) to analyze the differential expression of proteins in TDI and vehicle-treated lymph nodes.
Results: Body weight of control mice and TDI-treated mice did not differ. Interestingly, the weight of auricular lymph nodes significantly increased with an increasing concentration of TDI. 2D-DIGE analysis of these lymph nodes showed 35, 36 and 12 differential proteins comparing 0.3% and AOO, 3% TDI and AOO and 0.3% TDI and 3% TDI respectively. 17 proteins are already identified, among which several structural proteins and lymphocyte specific protein-1.
Conclusion: This study demonstrates the use of 2D-DIGE in the search for (early) biomarkers of chemical-induced asthma. Further studies will have to point out whether (some of) these proteins could act as valuable biomarkers. Therefore, more samples (bronchoalveolar lavage fluid, serum,…) will be analysed.
Publication status: accepted
KU Leuven publication type: AMa
Appears in Collections:Occupational, Environmental and Insurance Medicine (-)
Animal Physiology and Neurobiology Section - miscellaneous
Environment and Health - miscellaneous
× corresponding author
# (joint) last author

Files in This Item:

There are no files associated with this item.


All items in Lirias are protected by copyright, with all rights reserved.