Title: Coactivation of an endogenous progesterone receptor by TIF2 in COS-7 cells
Authors: Hofman, Kurt ×
Swinnen, Johan
Verhoeven, Guido
Heyns, Walter #
Issue Date: Jul-2002
Series Title: Biochemical and Biophysical Research Communications vol:295 issue:2 pages:469-74
Abstract: Transfection experiments, a powerful tool to study the function of steroid hormone receptors and their coregulators, are often performed in COS-7 cells, because of high transfection efficiencies and expression levels. Here we report on the presence in COS-7 cells of an endogenous steroid hormone receptor, which is highly responsive to progesterone and the synthetic steroids R1881 and ORG2058, but not to 5 alpha-DHT. A 10-fold excess of the progesterone antagonist RU486 abolishes the stimulation by progesterone, while cotransfection with the coactivator TIF2 increases its activity 6- to 7-fold. A comparison of the ligand specificity with transfected androgen or progesterone receptors indicates that the endogenous receptor is a progesterone receptor. Its presence is confirmed by steroid-binding experiments, RT-PCR and Northern blot analysis. Consequently, progesterone receptor function may be studied conveniently in COS-7 cells without cotransfection of receptor, but the endogenous receptor may interfere in studies of ligand specificity and coactivation of cotransfected receptors.
ISSN: 0006-291X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Clinical and Experimental Endocrinology
Laboratory of Lipid Metabolism and Cancer (+)
× corresponding author
# (joint) last author

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