Title: Clonal analysis and identification of epidemic strains of methicillin-resistant Staphylococcus aureus by antibiotyping and determination of protein A gene and coagulase gene polymorphisms
Authors: Hoefnagels-Schuermans, A ×
Peetermans, Willy
Struelens, M J
Van Lierde, S
Van Eldere, Johan #
Issue Date: Oct-1997
Series Title: Journal of clinical microbiology vol:35 issue:10 pages:2514-20
Abstract: Forty-three methicillin-resistant Staphylococcus aureus (MRSA) isolates with known genetic and epidemiological relatedness and different degrees of transmission were analyzed by antibiotyping, protein A gene polymorphism analysis, and coagulase gene polymorphism analysis. The three typing systems were evaluated for their performance and convenience to define clones and to discriminate between epidemic MRSA (EMRSA) and sporadic MRSA (SMRSA). Antibiotyping and AluI restriction fragment length polymorphism analysis of the coagulase gene were able to define clones in the same way as DNA macrorestriction analysis (SmaI). However, both techniques presented disadvantages, making neither of them useful as a single typing method. Protein A gene polymorphism analysis appeared to be of no value for clonal analysis. None of the three typing methods was able to differentiate between EMRSA and SMRSA.
ISSN: 0095-1137
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Academic Centre for Nursing and Midwifery
Laboratory for Experimental and Clinical Microbiology (-)
Laboratory for Clinical Infectious and Inflammatory Disorders
Hospital Hygiene (-)
Laboratory of Clinical Bacteriology and Mycology
× corresponding author
# (joint) last author

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