Docking studies on a new human immunodeficiency virus integrase-Mg-DNA complex: phenyl ring exploration and synthesis of 1H-benzylindole derivatives through fluorine substitutions
Ferro, Stefania De Luca, Laura Barreca, Maria Letizia Iraci, Nunzio De Grazia, Sara Christ, Frauke Witvrouw, Myriam Debyser, Zeger Chimirri, Alba # ×
Journal of Medicinal Chemistry vol:52 issue:2 pages:569-573
A new model of HIV-1 integrase-Mg-DNA complex that is useful for docking experiments has been built. It was used to study the binding mode of integrase strand transfer inhibitor 1 (CHI-1043) and other fluorine analogues. Molecular modeling results prompted us to synthesize the designed derivatives which showed potent enzymatic inhibition at nanomolar concentration, high antiviral activity, and low toxicity. Microwave assisted organic synthesis (MAOS) was employed in several steps of the synthetic pathway, thus reducing reaction times and improving yields.