Title: Anti-PlGF (Placental growth fractor) reduces choroidal neovascularization in a mouse model of age-related macular degeneration (ARMD)
Authors: Van de Veire, Sara ×
Vinores, S.
Peric, Aleksandar
Massimiliano, M.
Moons, Lieve
Stassen, Jean-Marie
Noel, A.
Stalmans, Ingeborg
Carmeliet, Peter #
Issue Date: 2008
Conference: ARVO Annual Meeting location:Fort Lauderdale, Florida, USA date:27 April - 1 May 2008
Abstract: Purpose:To evaluate whether and through which mechanism PlGF blockage can inhibit choroidal neovascularization (CNV)in a mouse model of ARMD.

Methods:CNV was induced in mice by placing 3 Argon laser burns on the choroid. In a first experiment we compared CNV formation between PlGF knock-out and wild type mice. Secondly, wild-type mice were injected intraperitoneally with 6, 12, 25 or 50 mg/kg of either an anti-PlGF-antibody, an anti-VEGF receptor-2 (VEGFR2) antibody, a combination of both or an irrelevant control antibody. The CNV lesions were evaluated on flat mounts and histological cross-sections. The amount of endothelial cells and inflammatory cells in the lesions was morphometrically analyzed after immunostaining for CD31 or F4/80 respectively. Thirdly, combination therapy with anti-PlGF and anti-VEGF receptor-2 was given to seek for additional inhibition of neovascularization.

Results:CNV formation was significantly reduced in PlGF knockout mice. Anti-PlGF significantly inhibited choroidal neovascularization, comparably to anti-VEGFR2 (p<0.05). Moreover, a combination treatment of anti-PlGF and anti-VEGFR2 further suppressed CNV (p<0.05). Finally, a significant reduction in the number of inflammatory cells was observed in the lesions treated with anti-PlGF (p<0.05), but not in anti-VEGFR2 treated mice.

Conclusion: Anti-PlGF treatment inhibits CNV formation in a mouse model of ARMD, and enhances the effect of anti-VEGFR2. Moreover, anti-PlGF reduces inflammation whereas anti-VEGF receptor-2 does not have any effect on the inflammatory pathway.
Publication status: published
KU Leuven publication type: IMa
Appears in Collections:Animal Physiology and Neurobiology Section - miscellaneous
Research Group Ophthalmology
Molecular Genetics Section (-)
Molecular and Vascular Biology
Vesalius Research Centre (-)
Laboratory of Membrane Trafficking
Laboratory of Angiogenesis and Vascular Metabolism (Vesalius Research Center) (+)
× corresponding author
# (joint) last author

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