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Title: Type III interferon-λ mRNA expression in sputum of adult and school-aged asthmatics
Authors: Bullens, Dominique ×
Decraene, Ann
Dilissen, Ellen
Meyts, Isabelle
De Boeck, Christiane
Dupont, Lieven
Ceuppens, Jan #
Issue Date: Oct-2008
Host Document: The European respiratory journal. Supplement vol:32
Conference: ERS annual Congress edition:18 location:Berlin date:October 4-8 2008
Abstract: Background: The increased susceptibility of asthmatics to rhinovirus infection has recently been related to deficient IFN-λ1 (IL-29) and IFN-λ2/3 (IL-28) production by bronchial epithelial cells and macrophages.
Objectives: We here studied IFN-λ mRNA expression in the airways of stable asthmatics in comparison to healthy subjects and in relation to asthma symptoms, non-invasive parameters of airway inflammation and lung function parameters.
Methods: Airway cells were obtained by sputum induction, in 14 healthy and 35 asthmatic adults and 12 asthmatic school-aged children. IFN-λ was studied at the mRNA level by quantitative RT-PCR.
Results: Asthmatic adults have increased sputum IL-28 mRNA and similar IL-29 mRNA expression in comparison to healthy subjects. In asthmatics, both sputum IL-28 and IL-29 mRNA expression correlate with the sputum CD3γ mRNA expression (reflecting infiltrated T cells) and IL-28 mRNA levels correlate with the percentage of sputum eosinophils. Sputum IL-29 mRNA (but not IL-28) correlates negatively with asthma symptoms in steroid naïve patients and is significantly higher in steroid treated than in steroid naive patients. Finally, both IL-28 and IL-29 mRNA levels are higher in asthmatic children than in asthmatic adults.
Conclusion: Our results show that asthmatic subjects have substantial type III Interferon-λ mRNA levels in the airways. Our data furthermore suggest that IL-29 could have an immunoprotective role in the lower airways.
Publication status: published
KU Leuven publication type: IMa
Appears in Collections:Laboratory of Pediatric Immunology
Pneumology
Laboratory of Clinical Immunology
× corresponding author
# (joint) last author

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