Title: Occurrence of autoimmunity after xenothymus transplantation in T-cell-deficient mice depends on the thymus transplant technique
Authors: Devos, Timothy * ×
Sprangers, Ben *
Lin, Yuan
Li, Shengqiao
Yan, Yehong
Landuyt, Willy
Lenaerts, Caroline
Rutgeerts, Omer
Goebels, Jozef
Bullens, Dominique
De Wolf-Peeters, Christiane
Mathieu, Chantal
Waer, Mark
Billiau, An #
Issue Date: Feb-2008
Publisher: Lippincott williams & wilkins
Series Title: Transplantation vol:85 issue:4 pages:640-644
Abstract: Xenothymus transplantation under the kidney capsule in athymic rodents frequently leads to multiorgan autoimmunity. Herein, we explore whether this is an intrinsic risk of xenothymus grafting or whether it depends on the transplant technique. We developed a new technique of "venous pouch" thymus grafting (heart-xenothymus) and compared this with the conventional kidney subcapsular technique (kidney-xenothymus) in a rat-into-nude-mouse model. Whereas lethal autoimmunity developed in 90% of kidney-xenothymus recipients, all heart-xenothymus grafted mice remained completely healthy. Autoimmunity in heart-xenothymus recipients was absent despite a significantly improved T-cell generation and was associated with significantly higher CD4(+)CD25(+) T-cell frequencies and CD4(+)CD25(+) cell Foxp3 mRNA levels than those observed in kidney-xenothymus recipients. In conclusion, we describe a novel vascular pouch technique of xenothymus transplantation that prevents the development of autoimmunity in nude mice. Our data further suggest that prevention of autoimmunity is related to a superior development of regulatory T-cells.
ISSN: 0041-1337
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Translational Cell & Tissue Research
Hematology Section (-)
Laboratory of Experimental Radiotherapy
Interface Valorization Platform (IVAP) (-)
Medical Clerkships Centers
Laboratory of Clinical Immunology
Laboratory of Experimental Transplantation
Clinical Residents Medicine
Laboratory of Nephrology
Laboratory of Pediatric Immunology
Clinical and Experimental Endocrinology
* (joint) first author
× corresponding author
# (joint) last author

Files in This Item:
File Description Status SizeFormat
Devos et al..pdf Published 1757KbAdobe PDFView/Open Request a copy

These files are only available to some KU Leuven Association staff members


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science