Polish Neuroscience Society - 8th International congress location:Krakau, Poland date:24-27 September 2007
The visual cortex of mammals remains vulnerable to changes in visual input throughout the entire lifespan. Monocular and binocular deprivation or retinal lesions induce specific alterations of function and connectivity. Although several molecules have been implicated in driving the growth and refinement of the cortical circuitry, the exact molecular framework that directs age- and experience-dependent plasticity remains to be determined. We exploit proteomics technology to compare protein expression profiles in cat visual cortex at eye opening (postnatal day 10 (P10), when experience-dependent plasticity starts), the peak of the critical period (P30), and in adulthood. Upon comparison of normal control subjects with monocularly or binocularly deprived animals or animals with retinal lesions it became clear that every manipulation has its characteristic molecular fingerprint. Moreover, the expression of molecules involved in developmental plasticity does not always follow the course of the critical period as previously acknowledged, and developmental and adult plasticity involve different protein expression patterns.
With this presentation we want to demonstrate the power of 2-D DIGE as a tool toward understanding the molecular basis of nervous system plasticity during development and adulthood.